1.
Libido And Mating Behaviour Of Buffalo Bulls
by Anzor, M | Manzoor Ahmed | Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0038,T] (1).
2.
Studiesw On The Senility Pattern In Relation To Reproductive Efficienty In Male Nili-Ravi Buffalo
by Farman Ahmad | Imtiaz Hussain Khan | Muhammed | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0039,T] (1).
3.
Accuracy Of Rectal Diagnosis Of Corpus Luteum In Nili Ravi Buffalo
by Subhan Qureshi, M | Imtiaz Hussain Khan | Muhammed | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1988Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0040,T] (1).
4.
Stuies On Superovulation And Morphology Of Recovered Embryos In Nili Ravi Buffaloes (Bubalus Bubalis)
by Tayyab Rahil | Chaudry Muhammed Saleem | Muhammed Aslam Bhatti | Saghir Ahhmed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1988Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0042,T] (1).
5.
Prevalence Of Brucellosis In Repeat Breeder Cows In Peshawar District (North West Frontier Province (N.W.F.P)
by Ali Nazar Bangash | Rashid Ahmed Chaudry | Muhammed | Naeem Ullah Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0044,T] (1).
6.
Effect Of Different Equilibration Times And Extenders On The Deep Freezing Of Buffalo Bull Semen
by Rashid, A | Imtiaz Hussain Khan | Muhammed | Naeem Ullah Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0050,T] (1).
7.
Studies On The Population Of Primordial Follicles In Teddy Goats
by Saeed Akhtar, M | Naeem Ullah Khan | Muhammed | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0053,T] (1).
8.
Studies On Reproductive Efficiency Of Bhagnari Cattle, Maintained At Govt. Cattle Farm Usta Muhammad Balochistan
by Azam, M | Rashid Ahmed Chaudry | Imtiaz Hussain | Muhammed Aslam Bhatti | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0088,T] (1).
9.
Sero Epidemiological Survey Of Bovine Brucellosis Associated With Reproductive Disorders In Quetta District, Balochistan
by Faqir Mohammad | Naeem Ullah Khan | Muhammed | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0089,T] (1).
10.
Sero Prevalence Of Leptospirosis In The Exotic Dairy Cattle In And Around Quetta
by Ashfaq Hussain, Bhatti | Imtiaz Hussain Khan | Muhammed | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0090,T] (1).
11.
Evaluation Of Dual Staining Procedure To Detect The Viability And True Acrosome Reaction Of Buffalo Bull Spermatozoa
by Munir Ahmad | Rashid Ahmed Chaudry | Muhammed Akram Muneer | Tayyab Rahil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0091,T] (1).
12.
Macro And Micro Elements In Female Buffalo Calves Blood Serum During Various Stages Of Growth Upto Maturity
by Sher Nadir | Naeem Ullah Khan | Imtiaz Hussain | Muhammed Yaqoob Malik | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: The study was carried out at Livestock Production Research Institute, Bahadarnagar, District Okara.
320 blood serum samples in duplicate from 64 female buffalo calves were chemically analysed for Calcium, Phosphorus, Copper, Zinc and Manganese.
These animals were divided into four groups and each group was further sub divided into two sub groups respectively. Eight animals were placed in each sub group. Sub groups of first group consisted of 5th and 6th, sub groups of second group 7th and 8th, sub groups of third group 9th and 10th, and sub groups of fourth group 11th and 12th quarters of their age respectively.
These animals were put on same plan of nutrition for thirty days before starting the experiment.
20 ml., blood was drawn aseptically from the jugular vein. The blood was allowed to coagulate and serum was separated for analysis. Inorganic phosphorus and copper were analysed in the blood serum by the Merko test kits, Calcium by flame photometer, Zinc and Manganese by atomic absorption spectrophotometer.
Biometry and ovarian activity of the third and fourth groups were recorded.
Non significant (P>O.05) difference was observed for calcium, phosphorus, copper and zinc in all the groups while significant (9<0.05) difference was existed for manganese in the groups.
significant correlation (r = 0.72* P <0.05) was observed for phosphorus with the size of the graafian follicle while correlation ( r = 0.62, r = 0.40 r=0.58 and r= 0.57 9>0.05) for calcium, copper, zinc and manganese was negative respectively.
Availability: Items available for loan: UVAS Library [Call number: 0209,T] (1).
13.
A Study To Determine The Time Of Ovulation In Relation To Oestrus In Nili-Ravi Buffalo
by Amjad Hussain | Rashid Ahmed Chaudry | Kazim Raza | Muhammed Khalid | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: A total of 32 Nili-Ravi cyclic buffaloes were selected for this study. Cyclicity was determined on the basis of presence of corpus luteum and was confirmed by luteal phase levels of progesterone. 10 animals were treated with PGF2-alpha to induce oestrus. The oestrus detection was made by teasing, behavioural symptoins, physical changes and rectal palpation. The receptivity of male was taken as a time for start of oestrus. The ovulation time was determined by repeated palpation and was confirmed by blood progesterone levels. The behavioural signs noted were; acceptance of teaser bull, frequent urination, raised tail, bellowing, restlessness, acceptance of mounting by other females, licking of external genitalia of other animals and loss of appetite. The physical changes recorded were; uterine tone, swollen vulva, mucous discharge on rectal palpation, absence of wrinkles on vulvular lips, hypraemia and wetness in the vagina. There was a little difference in the behavioural symptoms and physical changes among the groups. In the PGF2-alpha treated group the behavioural symptoms and physical changes were slightly more pronounced as compared to natural groups.
The length of oestrus in the natural groups was 23.5 ± 1.5 hours, whereas it was 27.2 ± 2.4 hours in the treatment group. The difference in the length of oestrus among the two groups was statistically significant. The ovulation time in he natural group was 34.6 ± 1.7 hours from the start of oestrus and 11.2 ± 0.5 hours from the end of oestrus. The ovulation time in animals treated with prostaglandin F2-alpha was 39.8 ± 2.9 and 12.6 ± 1 .2 hours in relation to the start and the end of oestrus, respectively. The difference in the ovulation time both in relation to the start and to the end of oestrus between the two groups was significant. The progesterone Values at the time of oestrus were 0. 1 ± 0.02 ng/ml and 0. 1 ± 0. 1 ng/ml in natural and treated groups respectively. And in the luteal phase (13), the progesterone values were 2.1 ± 0.2 and 2.6 ± 1.6 ng/ml in natural and treated groups respectively.
Availability: Items available for loan: UVAS Library [Call number: 0240,T] (1).
14.
Effect Of Different Extenders On The Extracellular Release Of Got And Gpt From Buffalo Bull Spermatozoa
by Naeem Iqbal | Imtiaz Hussain Khan | Muhammed | Naeem Ullah Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Preservation of spermatozoa in a solubrious environment has become an essential prerequisite to achieve the objectives of Artificial Insemination. Selection of suitable extender is based on evaluation of fresh and diluted semen through several parameters. In vitro, the most reliable and preferable method for the evaluation of semen preservation technique is the analysis of seminal plasma for several enzymes related with fertility, because, the extracellular concentration of these enzymes might serve as an index of membrane trauma. Among these enzymes, Glutamic Oxaloacetic Transaminase(GOT) and Glutamic Pyruvic Transaminase(GPT) are also used for the evaluation, because, they occur abundently in the spermatozoa and reflect their integrity. Moreover, they are easy to detect and play a major role in the protein and carbohydrate metabolism of spermatozoa. Some workers have evaluated extenders on these lines but this information was lacking in our condition, particularly in buffalo (Bubalus buballs).
For this purpose the semen was collected from ten buffalo bulls of Nili-Ravi breed maintained at Semen production Unit, Qadirabad. Semen was extended in three extenders namely, Lactose- egg yolk-glycerol (LEYG), Skim milk-egg yolk-glycerol (SMEYG) and Tris-egg yolk-glycerol (TEYG) and the GOT, GPT activities were measured in fresh semen, after extension, just after freezing and 30 days post-freezing. The data, thus, collected was subjected to statistical analysis.
The GOT activity (mU/ml) in fresh semen was 20.26±0.72 and in extender LEYG, the activity after extension, freezing and 30 days post-freezing was 38.06±2.09, 65.90±2.'2 and 80.94±2.78 respectively. The corresponding values in SMEYG and TEYG were, 51 .80±2.06 and 44.20±1.34, 90.22±1.79 and 74.38±2.30 and 109.10±2.14 and 87.68±2.33 respectively.
The GPT activity (mU/mi) in fresh semen was 7.98±0.30 and in LEYG after extension, just after freezing and 30 days' storage was 12.36±0.41, 18.52±0.62 and 22.20±0.76 respectively. These values for SMEYG and TEYG were, 13.96±0.60 and 13.28±0.56, 22.82±0.65 and 20.50±0.50 and 27.23±0.68 and 23.98±0.66 respectively. The overall GOT and GPT activities wee maximum in SMEYG (88.84 and 19.25) than in TEYG(67.42 and 16.00) and LEYG(60.68 and 14.22). In LEYG, upto freezing the release of GOT and:GPT was minimum but after 30 days' storage this situation was found in TEYG. Regarding the stages of freezing, maximum release of GOT, GPT was observed after freezing, i.e., 32.15 and 7.42 than after extansion (24.42 and 5.52) and after 30 days' storage(15.74 and 3.85 resp.).
Statistical analysis revealed significant (p/U.01) differences between the extenders SMEYG to LEYG and SMEYG to TEYG, but non-significant difference between LEYG and TEYG for the release of GOT and GPT. Highly significant (p/0.01) differences were existed Iethween all the stages of freezing.
The GPT and GOT ratio found in fresh semen was 1:2.53 and the average GPT, GOT ratio in SMEYG was 1:4.61, in LEYG 1:4.21 and It was 1:4.21 in TEYG extender. The GOT, GPT activities were found greater in young bulls as compared to older bulls. A significant correlation, i.e., -0.53 (p/0.05.) for GOT activity with age was found.
Regarding the relationship of motility percentage of spermatozoa with GOT, GPT release, a significant correlation(-0.44, p/0.05) between GOT release and motility percentage in TEYG extender after freezing was found. This correlation for GPT release in SMEYG after 30 days' storage was -0.69(p/0.0l), in TEYG after freezing 049 (p/0.05) and in same extender,. after 30 days' storage was -0.72 (p/U.01).
The average volume of both ejaculates had a significant relationship with GOT activity (r=0.55, p/0.05) and with GPT activity (r= -0.73, p/0.01) in fresh semen.
The overall maximum decline in motility was found in SNEYG (53.06%)than in LEYG (45.21%) and TEYG(44.27%). Statistically, the SMEYG had a significant (p/0.0l) decline in motility as compared to TEYG and LEYG,. while the difference between two latter extenders was non-significant. In all extenders, highly significant correlations (p/0.01) were existed between initial motility and motility recorded after extension, freezing and 30 days' storage.
While considering the results, the extenders LEYG and TEYG were found superior than the SMEYG, but TEYG was prefered on LEYG, due to maintaining high motility percentage of spermatozoa along with the minimal enzymes release and it can be used satisfactorily for the preservation of buffalo semen. Regarding the stages of preservation, the maximum enzymes were released after freezing than after extension and after one month storage in liquid nitrogen.
Availability: Items available for loan: UVAS Library [Call number: 0269,T] (1).
15.
Studies On The Postnatal Development Of The Reproductive Organs And Other Related Endocrine Glands In Male Nili
by Nasim Ahmad | Manzoor Ahmed | Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: The water buffalo is amongst the most productive of domestic animals. One of the major problems causing uneconomical productivity in buffalo has been described as its higher age of maturity. It was therefore, necessary to collect a through information from birth through puberty on the potential of anatomical and physiological development of its reproductive organs and endocrine glands. This information was particularly lacking on the male of Nili Ravi breed.
Ten male Nili-Ravi calves ranging in age from 0 to 7 days were included in the Present study. They were subjected to detailed clinical examination. Biometrical examination of reproductive organs accessory sex glands and endocrine glands was conducted after slaughtering. Samples from suitable places were taken for histology purpose. Necessary photography and microphotography was done and the data collected was statistically analysed.
Body weight, body temperature, respiration and pulse rate averaged 32.24 kg, 38.8° C, 27.6 and 78.2 per minute whereas heart girth, body length and scrotal circumference averaged 71.5, 75.0 and 8.38 cm. Coefficient of correlation among body weight and heart girth; scrotal circumference and total testicular weight was 0.96 (P<.0l), 0.69 (P<0.05) and 0.57(which was non-significanl. Correlations between scrotal circumference and total testicular veight was 0.65 (P<0.05).
Single testis measured 2.3 x 1.2 x 1.0 x 3,0 cm (LxBxTxC) and weighed 1.39 gm. Length of epididymis was 4.8 cm with a weight of 0.88 gm/vas deferens was 31.3 cm long and length of attached penis was 21.2 cm. Length and diameter of ampulla was 4.3x2.2 whereas length, breadth and thickness of seminal vesicle. Prostate and cowpers gland averaged 2.1xl.5x0.35, 5.6xl.7x1.6 and 0.6x0.3x0.2 cm. Seminal vesicle weighed 0.48 gm. Average measurements of pitutiary and pineal gland were l.3x0.8x0.6 and 0.5x0.5x0.3 cm. Their corresponding weights were 0.42 and 0.06 gm. There was slight difference in the measurements of right and left thyroid and adrenal gland. The difference between the weight of right and left thyroid and adrenal gland were significant (P>0.05).
Microscopic examination revealed that testis was composed of solid sex-cords which measured 45.04 and 47.83 microns in diameter near the periphery and near the rete. The ratio of large cells and small cell was 13:5 and of sex cords with interstitial tissue was 60:40. Epididymis exhibited moderate regional variation. Epithelial height was 14, 15 and 25 microns in head, body and tail regions.
Ampullary glands were devoid of cilia and showed dilatations Only towards the lumen. The glandular epithelium of seminal vesicle was columnar with granular cytoplasm. Their height varied from 15 to 20 microns. In prostate gland two parts were clearly distinguished. Secretory units of bulbouretheral gland were small and were lined by cuboidal epithelium. Penis of new born calf possesed all the essential structures.
In pituitary gland pars intermedia and median cleft was prominent and various cells of pars distails were identified. Pinealocytes were characterized as large cells situated more towards periphery.
Follicles of thyroid gland were lined by columnar cells and filled with colloid material. In adrenal cortex three zones were clearly identified and cortico-medullary junction was interdigitated.
The gross and histological findings of the present study were found to be little higher than those of Indian buffalo calves but were comparable or found lower when compared with cow calves of same age.
It is, proposed that similar studies be conducted on Nili-Ravi calves of different age groups uptil puberty to observe the develop- mental changes. The recorded variations could then be narrowed to select sires showing early maturity for exploitation through AL. to over come the problem of delayed maturity.
Availability: Items available for loan: UVAS Library [Call number: 0274,T] (1).
16.
Studies On Postpartum Buffaloes - Changes In Reproductive Organs And Onset Of Oestrus After Parturition
by Munir Ahmnad Ch | Naeem Ullah Khan | Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1985Dissertation note: Thirty five (35) buffaloes of Nili-Ravi breed were studied for postpartum reproductive performance. Involution of the uterus and postpartum ovarian activity were monitored by repeated rectal palpations of the genitalia from day 14 postpartum until first observed oestrus. Buffaloes were checked for manifestation of oestrus for five times a day by using bulls with deviated penis. Buffaloes detected in oestrus were examined rectally and inseminated at 12 and 18 hours after the onset of oestrus.
The mean time required for complete involution of uterus was 27.5 ± 1.4 days with a range from 21 to 66 days. The rate of decrease in diameter from day 14 postpartum to complete involution was slower in non-gravid horn than in gravid horn. This overall uterine involution period was not affected by the month of calving and the age of the buffaloes but lactation number effected it significantly (P<0.06). The diameter of cervix on day 14 postpartum was 88.0 mm for buffaloes calving during March and ranged from 66 to 77 mm for buffaloes calving during other months of the study period. The rate of decrease from day 14 postpartum to complete involution was lower in buffaloes which calved in January (1.00 mm/day) as compared to buffaloes of other months (range 1.1 to 1.5 mm/day). On complete involution the diameter of cervix was 47.0 mm and it ranged 34 to 37 mm in other months of study. There was no effect of age and parity on rate of cervical involution. Similarly the rate of decrease from day 14 postpartum to complete involution, of the gravid and non-gravid horns was not effected by month of calving, age and parity of buffaloes.
The time required from calving to complete regression of corpus luteum was 20.6 ± 1.0 days. This period was found to be effected by the month of calving (P<0.06). The interval from calving to the formation of first palpable follicle averaged 27.4 ± 1.2 days. There was significant effect of resumption of follicular activity on formation of first corpus luteum (P<0.001) and first postpartum oestrus (P<0..001). The incidence of first corpus luteum formation without oestrus activity was 62.9 %. The palpable life span of first corpus luteum averaged 6.6 ± 0.7 days. The interval from calving to formation of first corpus luteum was 38.1 ± 1.5 days. It was significantly affected by the postpartum oestrus interval (P<0.009)
The average postpartum period from calving to first observed oestrus was 124.2 ± 14.5 days and ranged from 22 to 280 days. In 22.9 % buffaloes, postpartum oestrus was observed within 30 days, whereas in 57.1 % buffaloes this period was more than 120 days. This interval was highly correlated with interval to uterine involution (P<0.06) and duration of oestrus (P<0.06) . Onset of oestrus was most frequently i.e in 34.3 % buffaloes were obsered at 22.00 hours, followed by 18.00, 02.00, 06.00 and 12.00 hours in 22.9 %, 17.1 %, 14.3 % and 11.4 % buffaloes, respectively.
In contrast to onset, the termination of oestrus occurred more frequently (77.1 %) during day light hours (6 AM to 6 PM) and 6.00 PM was the time when majority of buffaloes (40 %) terminated their oestrus. The overall duration of first postpartum oestrus was 24.7 ± 1.6 hours and it ranged from 6 to 48 hours. The majority of buffaloes (65.8 %) had the duration of oestrus within 9 to 24 hours.
On the basis of 60 days pregnancy diagnosis, the conception rate to breeding at first oestrus was only 14.30%.
Availability: Items available for loan: UVAS Library [Call number: 0276,T] (1).
17.
Comparative Studies On The Therapeutic Effect Of Cloprostenol And Antibiotics On Endometritis In Buffaloes
by Latif, M | Rashid Ahmed Chaudry | Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: This study was designed to test suitable drugs on the basis of their theraputic effect which are currently in practice to treat endometritis, a problem of high significance among the reproductive disorders of the buffalo in Pakistan.
One hundred and twenty Nili-Ravi buffaloes suffering from 1st degree of endometritis were used in this study. Six groups were constituted, having 20 animals in each group.
Twenty Nili-Ravi buffaloes in group A' were injected 500 mg of cloprostenol (Estrumate) and inseminated at the following oestrous.
Twenty Nili-Ravi buffaloes included in group 'B' were treated with oxytetracycline for five days using intra-uterine route. On next heat the buffaloes were inseminated.
Twenty buffaloes of group 'C' were treated with oxytet-racycline, opted single dose after intrarectally palpating the corpus luteum on the ovary. The buffaloes were inseminated on next heat
Twenty buffaloes of group 'D' were treated with Chioramphenicol for five days using intra-uterine route. The animals were inseminated on next heat
Nili- Ravi buffaloes included in group 'E' were treated with Chloramphenicol after palpating the corpus luteurn. opting single dose. The animals were inseminated on next heat.
The Nili-Ravi buffaloes included in Group 'F' were used as control and inseminated without treatment. During the fertility trials the conception rate calculated after rectal-palpation for pregnancy diagnosis was 60%, 55%, 45%, 50%, 40% and 35% for groups A, B, C, D, E and F respectively.
On the basis of conception rate percentage(60%) and services per conception (1.6) estrumate was declared as drug of choice1,among these tested in the present study.
Availability: Items available for loan: UVAS Library [Call number: 0320,T] (1).
18.
Effect Of Different Egg Yolk Levels On The Quality Of Frozen Buffalo Bull Semen
by Afzal, M | Rashid Ahmed Chaudry | Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: The comparative study of various egg yolk levels in the extenders used for deep freezing of buffalo semen was conducted at Semen Production Unit, Qadirabad (Sahiwal). The routinely used Lactose-Fructose-Glycerol semen extender was added with 20, 15, 10, 5 and 0% egg yolk concentration in five experimental extenders. 50 ejaculates collected from 10 buffalo bulls were frozen in Liquid Nitrogen. Each semen sample from all bulls and egg yolk levels was evaluated for post-thaw sperm motility, livability and absolute index of livability.
The average values obtained from egg yolk levels and bulls for all test parameters were found statistically high (P < 0.01) at 10 percent egg yolk level as compared to all others. The order of merit for post-thaw motility, livability and the absolute index of livability at 10, 20, 15, 5 and 0% egg yolk level was 45.9%, 43.5%, 42.9%, 33.7% and 8.7% for motility, 9.8, 9.34, 9.3, 7.68 and 2.75 (Hours) for livability and 231.6, 207.95, 204.85, 123.25 and 13.85 for livability index, respectively.
The comparative mean values obtained from each bull were observed non-significant for post-thaw sperm motility but were found significantly higher (P < 0.05) among bulls regarding livability and the absolute index of livability.
On the basis of the results obtained in the present study it may be concluded that due to synergestic effect between glycerol and the presence of 10% concentration of egg yolk in the extender, being comparatively more economical, exhibiting better visibility and on account of high values obtained from motility, livability and absolute index of livability, an extender containing 10% egg yolk level is recommended for the preservation and deep freezing of buffalo bull semen in Liquid Nitrogen for obtaining optimal semen quality.
Availability: Items available for loan: UVAS Library [Call number: 0322,T] (1).
19.
Studies On Quantity & Quality Of Semen Produced By Friesian Bulls In Subtropical Conditions
by Majid, A | Imtiaz Hussain Khan | Muhammed | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: Data of spermatological traits of 3490 ejaculates obtained during four years (1987 to 1990) from ten Friesian bulls were investigated to see the effects of age, seasons and bulls upon different seminal attributes. The age-wise overall mean values of different attributes of these bulls were: age and body weight at first ejaculation 329.7 days and 337.1 kilograms, number of ejaculates 7.41, ejaculate volume 4.08 ml, mass motility 1.14 grades, initial motility 51.43 per cent, sperm cells concentration 601.09 millions per ml, discarded ejaculates 3.17 and doses produced per ejaculate 32.66 number.
Age exerted highly significant effect (P<0.01) upon number of ejaculates, ejaculate volume, sperm concentration, ejaculate discarded and doses per ejaculate. A significant effect (P<0.05) was observed on percentage motility. There was a non-significant effect on moss motility. The 4th year was proved to be the best age for semen production because mean values of three seminal characteristics namely number of ejaculates, ejaculate volume and percentage motility were maximum during this year. Minimum mean ejaculates were also discarded during the 4th year.
Second year was observed as the worst year for semen production. Minimum mean values of number of ejaculates, ejaculate volume and percentage motility were observed in this year. Maximum mean ejaculates were also discarded during second year of age.
Seasons exerted highly significant effect (P<0.01) upon mass motility, percentage motility, sperm concentration, discarded ejaculates and doses per ejaculate. A significant effect (P<0.05) of seasons was observed upon number of ejaculates but the effect on ejaculate volume was non-significant (P>0 05)
The winter was proved to be the best semen producing season, because two seminal characteristics i.e. mass motility and pci centage motility had maximum mean values in this season Minimum mean ejaculates were also discarded in winter season The second best season was spring, in which ejaculate volume and doses per ejaculate were at their highest mean values
The worst season for semen production was humid hot season. Four seminal attributes i.e. number of ejaculates, mass motility, percentage motility and sperm concentration had minimum mean values in this season. Maximum mean ejaculates were also discarded in humid hot season. The second worst season was autumn. Only mean ejaculate volume and doses per ejaculate were at their lowest margin in autumn.
Highly significant effect (P<0.01) of bulls was observed upon ejaculate volume, mass motility and sperm concentration. Significant effect (P<0.05) of bulls was recorded for initial percentage motility and semen doses per ejaculate. The effect of bulls upon number of ejaculates and ejaculates discarded was found to be non-significant (P>0.05).
Bull No. F 16 proved to be the best bull for semen quantity and quality withmaximum number of ejaculates and maximum ejaculate volume. F 16 was also good in percentage motility, sperm cell concentration and doses per ejaculate. Moreover minimum ejaculates were discarded of F 16 too.
Bull No. 10 was the worst bull. Lowest values were observed in number of ejaculates, percentage motility and sperm concentration. F 10 was also not good in mass motility and maximum ejaculates were discarded from this bull.
The principle conclusion of this study is that if appropriate steps are taken to mitigate the effects of climate and high level of management practices is maintained, there appears to be no reason why high ambient temperatures and humidities should be inimical to successful semen production with Friesian bulls in the subtropical environments
Availability: Items available for loan: UVAS Library [Call number: 0350,T] (1).
20.
Seasonal Variation In Breeding Patterns Of Buffalo In Punjab
by Col. Ahmed Khan | Dr. Imtiaz Hussain Khan | Dr. Muhammed | Prof.Dr. Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: Buffalo plays a pivotal role in the economy of poor and landless farmers. Seasonal calving trend of buffalo has caused shortage of milk during the Summer season when it is required more. This study was undertaken to determine the effect of season on reproductive and productive traits of buffaloes.
In this project Artificial Insemination data of 5 Districts of Central Punjab and breeding data of the Livestock Experimental Station, Rakh Dera Chahi, Lahore were used. Analysis of variance was applied to study the effect of season on Artificial Insemination and Farm data.
Data analysis indicated a significant effect of season on breeding, fertility and calving. Both Artificial Insemination and Farm data indicated high (P<0.05) breeding trend during Autumn and Winter season. Fertility were significantly (P<0.05) low during Autumn. There was no significant effect (P>0.05) of calving season on the calving interval and milk production.
The conclusion revealed that the provision of ideal management, avoiding the extreme weather conditions, ensuring the availability of balanced adequate ration throughout the year and protection from other stresses of diseases etc., it may be possible to derive all the productive and reproductive traits from this versatile animal round the year without any significant effect of the various seasons.
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21.
Effect Of Osmotic Pressure On The Membrane Integrity Of Frozen-Thawed Buffalo Bull Spermatozoa
by Muhammed Irfan-Rehman Khan | Prof. Dr. Ijaz Ahmed | Dr.Azhar | Dr.Muhammed Mushtaq | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2005Dissertation note: In the first experiment, semen from seven Nili-Ravi buffalo bulls was used to study plasma membrane integrity of freshly collected (raw) and frozen-thawed sperm using the hypo-osmotic swelling test (HOST). For this purpose, percentage motility, integrity of plasma membrane and acrosome was assessed by a phase contrast microscope, HOST plus eosin-nigrosin staining and normal apical ridge test. respectively. 50fJI each of raw and frozen-thawed semen was mixed with 500fJI of 50, 100, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37°C for 1 h. Integrity of sperm plasma membrane was assessed before and after hypo-osmotic treatments to estimate the extent of damage for each hypo-osmotic treatment. In raw semen, the number of swollen sperm was higher (P<0.05) at 50, 100, 150 and 190 mOsm as compared to 250 mOsm. A positive but non-significant correlation (P>0.05) was found between percentage of swollen and live sperm at 100, 150, 190 mOsm. Similarly, a positive but non-significant correlation (P>0.05) was found between percentage of swollen sperm and motility at 50, 100, 150, 190 and 250 mOsm. In frozen-thawed semen, the number of swollen sperm w.as higher (P<0.05) at 50 and 100 mOsm as compared to 150, 190 and 250 mOsm and this number decreased significantly (P<0.05) and gradually from 82.6±5.99 at 150 mOsm to 69.7±5.49 at 190 mOsm and 42.6±4.07 at 250 mOsm. A positive but non-significant correlation (P>0.05) was found between percentage of swollen and live sperm and between percentage of swollen sperm and motility at 50, 100 150, 190 and 250 mOsm. The number of sperm with intact acrosome did not differ (P>0.05) in raw and frozen-thawed semen among treatments. Percentag~ motility in raw semen was highe~' (81%) as compared to frozen-thawed semen (60%). Damage to plasma membrane'was higher (P<0.05) at 50 mOsm (59% raw vs. 70% frozen-thawed) as, compared to other hypo-osmotic treatments, while minimum damage occurred at 250 mOsm (4.1% raw vs. 9.7% frozen-thawed). In the second experiment, hypoosmotic swelling test (HOST) plus eosin-,nigrosin staining and normal apical ridge test (NAR) were used to determine integrity of plasma membrane and acrosome of raw, diluted (cooled to 5°C) and frozen-thawed sperm. Semen from seven bulls was used in this study. For diluted and frozen-thawed sperm, three straws were pooled at 37°C. Percentage motility of raw, diluted and frozen-thawed sperm was assessed using a phase contrast microscope. 501-11 each of raw, diluted and frozen-thawed sperm was mixed with 5001-11 of 50, 100, 150, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37°C for 1 h. Total number of intact (live) sperm of raw, diluted and frozen-thawed semen was assessed before HOST. Diluted sperm showed higher (P<0.05) swellings of plasma membrane at 50
and 100 mOsm than raw sperm. Similarly, swellings of diluted sperm were' higher (P<0.05) than frozen-thawed sperm. Swellings of raw sperm were significantly higher (P<0.05) at 100, 150, 190 and 250 mOsm than frozenthawed sperm. A significant decrease (P<0.05) was found among percentage motility of raw (81±1.57), diluted (69.6±2.24) and frozen thawed (60.1 ±1.34) sperm. Live sperm were higher (P<0.05) in raw (174.4±7.33) and diluted semen (175.6±3.76) as compared to frozen-thawed semen (142.3±4.84). Although integrity of the acrosome of raw, diluted and frozen-thawed sperm did not differ (P>0.05), significan~ variation was found within bulls. In conclusion, fresh and frozen-thawed sperm behaved differently to HOST and the number of swollen sperm was higher in raw as compared to frozenthawed semen. Plasma membrane' integrity of raw and diluted sperm was compromised during freezing and thawing. However, freezing had no effect on acrosome integrity. Moreover, 100, 150 and 190. mOsm were found suitable to perform HOST.
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